Define queue. queue synonyms, queue pronunciation, queue translation, English dictionary definition of queue. n. 1. A line of waiting people or vehicles. 2. queue meaning, definition, what is queue: a line of people, usually standing or in cars, waiting for something. Learn more. Here is the difference: Cue: hint (I enjoy using it as 'taking cue from');. Queue: line , row. Que: It is not English word. It is short form of Quebec.